Genotyping and primer details


Genotyping and primer details for SALK_127430c - At2g20580
Line ID SALK_127430c
Locus At2g20580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8862737 of line SALK_127430c Chr2:8862737
   
Insertion allele amplicon LBb3 - 63qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63qz: 5'-ATGAATTATGTCACTCAACCACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8863260
   
wt amplicon (over InsPos) 10rz - 63qz
wt amplicon start Chr2:8862497
wt amplicon end Chr2:8863260
wt amplicon size 764 bp
Locus specific primer 63qz: 5'-ATGAATTATGTCACTCAACCACCA-3'
2nd locus specific primer 10rz: 5'-TAAGAACGATTGTGATCCTGTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11