Genotyping and primer details


Genotyping and primer details for SALK_127790c - At5g07890
Line ID SALK_127790c
Locus At5g07890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2519342 of line SALK_127790c Chr5:2519342
   
Insertion allele amplicon LBb3 - 31ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31ty: 5'-TGCTCGGATTTAGACATACACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2518771
   
wt amplicon (over InsPos) 16uu - 31ty
wt amplicon start Chr5:2518771
wt amplicon end Chr5:2519815
wt amplicon size 1045 bp
Locus specific primer 31ty: 5'-TGCTCGGATTTAGACATACACAAC-3'
2nd locus specific primer 16uu: 5'-TGAAATTGGCTATCTAGTGGTGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11