Genotyping and primer details

Genotyping and primer details for SALK_128170 - At3g20000
Line ID SALK_128170
Locus At3g20000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6969462 of line SALK_128170 Chr3:6969462
Insertion allele amplicon LBb3 - 13jd
Locus specific primer 13jd: 5'-CAGGGAAAATATGGCAATGTTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6969932
wt amplicon (over InsPos) 75rd - 13jd
wt amplicon start Chr3:6969139
wt amplicon end Chr3:6969932
wt amplicon size 794 bp
Locus specific primer 13jd: 5'-CAGGGAAAATATGGCAATGTTACT-3'
2nd locus specific primer 75rd: 5'-GTGTAGTCAAATAGCCAGGGAAAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11