Sequences from confirmation amplicon


Confirmation sequences for line SALK_128170 and hit At3g20000 are shown.
Line ID SALK_128170
Locus At3g20000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6969462
Primers used for PCR 13jd - LBb3, show primer details
   
Sequence Name 39-K115696-SALK_128170C-13jd
GenBank Accession LN485280 [GenBank]
Sequencing primer gene specific primer, 13jd: 5'-CAGGGAAAATATGGCAATGTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115696-SALK_128170C-13jd
TACATCTCTTGAAAGTAGTTGTACATGAAATCAGTGGCAAGTGACACCTGTGTGTCATGA
TTCATGAAAAAGATTGAATGTGTCATGATATATCTCTCAACTCTACAAGGTTTTAAGATG
CAATCAAATGAGAACGGTACATCACCTTATCTGAAATCTTCTGAACATAGTTCATAACAA
CAGCACCCGTGCTAGCAACTTGACCAGAAGCAACCTGTTCATCAAGAACAGTTCTTAATA
TGATGGGTATATAAACTCAAAGTGTTCAAAATTCTGATCTACTTTCAGCTAACTGAGTTT
TTGAACCTTGCAATTTGTATAATAAACCAACTGAGATGAAGCACTACCATTTTATCTGTC
TCGTATCTAGCAGCATAGCCTATTCCCGACTTCCGAGGCACACCAGCAGCAAGCGGACGT
TTATATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCGA
   
Sequence Name 28-K115695-SALK_128170C-R210
GenBank Accession LN485091 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115695-SALK_128170C-R210
ACGTCCGCTTGCTGCTGGTGTGCCTCGGAAGTCGGGAATAGGCTATGCTGCTAGATACGA
GACAGATAAAATGGTAGTGCTTCATCTCAGTTGGTTTATTATACAAATTGCAAGGTTCAA
AAACTCAGTTAGCTGAAAGTAGATCAGAATTTTGAACACTTTGAGTTTATATACCCATCA
TATTAAGAACTGTTCTTGATGAACAGGTTGCTTCTGGTCAAGTTGCTAGCACGGGTGCTG
TTGTTATGAACTATGTTCAGAAGATTTCAGATAAGGTGATGTACCGTTCTCATTTGATTG
CATCTTAAAACCTTGTAGAGTTGAGAGATATATCATGACACATTCAATCTTTTTCATGAA
TCATGACACACAGGTGTCACTTGCCACTGATTTCATGTACAACTACTTTTCAAGAGATGT
AACAGCTAGTGTGGGTTATGACTATATGCTTAGACAGGTAAGTAACATTGCCTATTTTTC
CCT

Last Updated on 06.12.2018 16:11