Genotyping and primer details


Genotyping and primer details for SALK_128365 - At3g02730
Line ID SALK_128365
Locus At3g02730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:588612 of line SALK_128365 Chr3:588612
   
Insertion allele amplicon R204 - 02ah
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 02ah: 5'-GAGAAGAAGGCTGGTGATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:587504
   
Insertion allele amplicon LBb3 - 03ah
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03ah: 5'-TCAAAGTTTCCGTGGCTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:589153
   
Insertion allele amplicon LBb3 - CG68
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG68: 5'-TACGACGACGTTGTGTTTCTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:588936
   
wt amplicon (over InsPos) CG68 - 02ah
wt amplicon start Chr3:587504
wt amplicon end Chr3:588936
wt amplicon size 1433 bp
Locus specific primer 02ah: 5'-GAGAAGAAGGCTGGTGATCG-3'
2nd locus specific primer CG68: 5'-TACGACGACGTTGTGTTTCTAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11