Genotyping and primer details


Genotyping and primer details for SALK_128444 - At4g30860
Line ID SALK_128444
Locus At4g30860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15024509 of line SALK_128444 Chr4:15024509
   
Insertion allele amplicon R204 - BI48
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI48: 5'-TCCTAAAATCCAAATCCCATGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15024985
   
Insertion allele amplicon LBb3 - BI49
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BI49: 5'-CATATCCAAAAACGAAAACGAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15024143
   
wt amplicon (over InsPos) BI49 - BI48
wt amplicon start Chr4:15024143
wt amplicon end Chr4:15024985
wt amplicon size 843 bp
Locus specific primer BI48: 5'-TCCTAAAATCCAAATCCCATGTAG-3'
2nd locus specific primer BI49: 5'-CATATCCAAAAACGAAAACGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11