Sequences from confirmation amplicon


Confirmation sequences for line SALK_128444 and hit At4g30860 are shown.
Line ID SALK_128444
Locus At4g30860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15024509
Primers used for PCR BI48 - R204, show primer details
   
Sequence Name 70-K105066-SALK_128444C-BI48
GenBank Accession LN485994 [GenBank]
Sequencing primer gene specific primer, BI48: 5'-TCCTAAAATCCAAATCCCATGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K105066-SALK_128444C-BI48
CCAAGTCATTAATTTGACATAACAAACAACATACCATTTTCTTGGCACCAACAAGAAAAG
GAAGGAAACATGTCGACTCTGAAACACCAGAAGCCACTCTTCTCTTGACCCAATCCTTCA
CATGATCATCCAAGACAAACCCTTTCTTGACTCTGTCAGACGGATTCTGTTTCCGGGTAA
AAGCAGTGACTCCATTAGCAATTTTAATGTCCTTGACCTCTTCCTCCGGTGGAATCATTG
GTAAATGTTTCCCATTGCATTCTCCGGCTAGATTTTCCGGGGAATTGATAGATTTGGCTA
ATTCAGGGCCTGAAGCCGCCGGAAGAAAAGAAGGGCAACAAGTGAGAGCAACAGATGCAG
ACATAGACATGTTTCCCAGACAACTTAATAACACATTACGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCGTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAACGGNTCCAC
   
Sequence Name 72-K105067-SALK_128444C-R210
GenBank Accession LN486045 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K105067-SALK_128444C-R210
GTCTTGTCTGCATCTGTTGCTCTCACTTGTTGCCCTTCTTTTCTTCCGGCGGCTTCAGGC
CCTGAATTAGCCAAATCTATCAATTCCCCGGAAAATCTAGCCGGAGAATGCAATGGGAAA
CATTTACCAATGATTCCACCGGAGGAAGAGGTCAAGGACATTAAAATTGCTAATGGAGTC
ACTGCTTTTACCCGGAAACAGAATCCGTCTGACAGAGTCAAGAAAGGGTTTGTCTTGGAT
GATCATGTGAAGGATTGGGTCAAGAGAAGAGTGGCTTCTGGTGTTTCAGAGTCGACATGT
TTCCTTCCTTTTCTTGTTGGTGCCAAGAAAATGGTATGTTGTTTGTTATGTCAAATTAAT
GACTTGTGAAGTTTTTAGATTTATTGACATATAGACTACATGGGATTTGGATTTTAGG
Primers used for PCR BI49 - LBb3, show primer details
   
Sequence Name 53-K119290-SALK_128444C-BI49
GenBank Accession LN485592 [GenBank]
Sequencing primer gene specific primer, BI49: 5'-CATATCCAAAAACGAAAACGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K119290-SALK_128444C-BI49
TGATCATACGATATACTTTCAAATCATGATTTGATTAATTTTAGCTATTTGTTCAGTTCC
GTTTTGGGAACCGGCATTAATCATTGCGTGTAGCACTGTTATTGGTAGATATGAATGTGG
GGGATTTTAGTTGGTAAAGAAATTTAACTAGCAGAAAAAATGTTTGGGGGCAATTAAAAC
AATATTTAAAATACAAGGACCTTTTTGTATAATATTTTAGGACAAATCTCCCGCCTTAAA
TTCCCTCCATATTTTTTCATGTCTCTTCGCTTTTCGCCTACCCAACAAAGACAAAACGTA
ACTGTCTTCTTCTATGTCTATGTCTACATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCAAA
   
Sequence Name 05-K119289-SALK_128444C-R210
GenBank Accession LN484418 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119289-SALK_128444C-R210
AGAAGAAGACGTTACGTTTTGTCTTTGTTGGGTAGGCGAAAAGCGAAGAGACATGAAAAA
ATATGGAGGGAATTTAAGGCGGGAGATTTGTCCTAAAATATTATACAAAAAGGTCCTTGT
ATTTTAAATATTGTTTTAATTGCCCCCAAACATTTTTTCTGCTAGTTAAATTTCTTTACC
AACTAAAATCCCCCACATTCATATCTACCAATAACAGTGCTACACGCAATGATTAATGCC
GGTTCCCAAAACGGAACTGAACAAATAGCTAAAATTAATCAAATCATGATTTGAAAGTAT
ATCGTATTGATCAGTGTTCGGTTTGTTAGTTATAAACAAGATATTTCGTTTTCGTTTTTG
GATATG

Last Updated on 06.12.2018 16:11