Genotyping and primer details


Genotyping and primer details for SALK_128723c - At1g05620
Line ID SALK_128723c
Locus At1g05620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1680838 of line SALK_128723c Chr1:1680838
   
Insertion allele amplicon LBb3 - 27rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27rb: 5'-AGACTTTCTCATCCGCAATTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1680310
   
wt amplicon (over InsPos) 47sq - 27rb
wt amplicon start Chr1:1680310
wt amplicon end Chr1:1681258
wt amplicon size 949 bp
Locus specific primer 27rb: 5'-AGACTTTCTCATCCGCAATTTTTA-3'
2nd locus specific primer 47sq: 5'-ATACCACTTGTCTGCACTCTAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11