Genotyping and primer details


Genotyping and primer details for SALK_128874c - At3g49420
Line ID SALK_128874c
Locus At3g49420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18329934 of line SALK_128874c Chr3:18329934
   
Insertion allele amplicon LBb3 - 29ie
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ie: 5'-GCCAAATGAACACACATAGATGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18330490
   
wt amplicon (over InsPos) 02km - 29ie
wt amplicon start Chr3:18329845
wt amplicon end Chr3:18330490
wt amplicon size 646 bp
Locus specific primer 29ie: 5'-GCCAAATGAACACACATAGATGAC-3'
2nd locus specific primer 02km: 5'-TACAGGAACAATGATGTCCTTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11