Sequences from confirmation amplicon


Confirmation sequences for line SALK_128874c and hit At3g49420 are shown.
Line ID SALK_128874c
Locus At3g49420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18329934
Primers used for PCR 29ie - LBb3, show primer details
   
Sequence Name 26-K113259-SALK_128874C-29ie
GenBank Accession LN485035 [GenBank]
Sequencing primer gene specific primer, 29ie: 5'-GCCAAATGAACACACATAGATGAC-3'
Sequence (A. th genome BLAST matches underlined)
>26-K113259-SALK_128874C-29ie
CAGAACTGGCCCAAGCTATTGATAATGTAGACAAAGACAGAAACAAACCTTATAATTTTG
ACGCTTCATGAAGAACTGCATGGTAGACTTGAAGCCAATGGTTAGACTAACCCCCGAGAT
GAAAAGAATCTGAGAAAATATGAAAAGAAACAAGCTCTCATACTAGTTCTGTACGTGTAT
GCAATAATAGTGGTCAAAGTATAGAAACAAAGATACATGAGATACTTACATTTCCCATGG
CAAGTAATCCCTTATCAAAGACGAAGACTATTCCCAAAAACGAGAAGAATACGCCAAATC
CTGTCAGGCCTAACCCGATTTCTGTTTCAAATCACATAAGAAACGTTTAAGTATATCCAA
GTACAGCATGAGTAACTTCGTAACCTGGTAATAAGATATACTGAAACTAAATGGGGTTCC
GAACAACAACATGACATTGAATGATCTACTAACTCAAGAACGTGATAAGCAAAAGAAGAA
TTAAGTATAACTAAGTAACAGGTATAAACAAATTGAAGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 09-K113258w-SALK_128874C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined)
>09-K113258w-SALK_128874C-R210
TTTGTTATACCTGTTACTTAGTTATACTTAATTCTTCTTTTGCTTATCACGTTCTTGAGT
TAGTAGATCATTCAATGTCATGTTGTTGTTCGGAACCCCATTTAGTTTCAGTATATCTTA
TTACCAGGTTACGAAGTTACTCATGCTGTACTTGGATATACTTAAACGTTTCTTATGTGA
TTTGAAACAGAAATCGGGTTAGGCCTGACAGGATTTGGCGTATTCTTCTCGTTTTTGGGA
ATAGTCTTCGTCTTTGATAAGGGATTACTTGCCATGGGAAATGTAAGTATCTCATGTATC
TTTGTTTCTATACTTTGACCACTATTATTGCATACACGTACAGAACTAGTATGAGAGCTT
GTTTCTTTTCATATTTTCTCAGATTCTTTTCATCTCGGGGGTTAGTCTAACCATTGGCTT
CAAGTCTACCATGCAGTTCTTCATGAAGCGTCAAAATTATAAGGTTTGTTTCTGTCTTTG
TCTACATTATCAATAGCTTGGGCCAGTCTCTGCTGGATTTTTACAGATTTCTACTTAATG
TCATCTATGTGTGTCCATTTGG

Last Updated on 06.12.2018 16:11