Genotyping and primer details


Genotyping and primer details for SALK_129208c - At2g17780
Line ID SALK_129208c
Locus At2g17780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7726159 of line SALK_129208c Chr2:7726159
   
Insertion allele amplicon LBb3 - 31td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31td: 5'-AAACAGAATGAGAGGGCTTACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7725816
   
wt amplicon (over InsPos) 16ua - 31td
wt amplicon start Chr2:7725816
wt amplicon end Chr2:7726469
wt amplicon size 654 bp
Locus specific primer 31td: 5'-AAACAGAATGAGAGGGCTTACAAA-3'
2nd locus specific primer 16ua: 5'-CAGGAGAGGCTAGAAGCTATTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11