Sequences from confirmation amplicon


Confirmation sequences for line SALK_129208c and hit At2g17780 are shown.
Line ID SALK_129208c
Locus At2g17780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7726159
Primers used for PCR 31td - LBb3, show primer details
   
Sequence Name 78-K116811-SALK_129208C-31td
GenBank Accession LN486197 [GenBank]
Sequencing primer gene specific primer, 31td: 5'-AAACAGAATGAGAGGGCTTACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K116811-SALK_129208C-31td
TCAGCAGTCGGCGTGCCATTTCACGGTGTTGCAATGATCTTCCCGAAAGTAGTTCATGTC
CGGATGAAACATAAGACGTTGATCTACATTCAACCATTAGACACCAATCAAACATTAGCC
TTCAATGATCAAACGTGAACAACACGTTTTGTGAGAACACATTAAGTATATGAATATTTT
CATCTATATCGGAGTAAATGGGATTAACCTTGAATCTGCTCGAATACTACTACTATCCGT
GTCATACAAATCATCTCTCTTCTTGCTAGAGGTAAGCTCTTCTTTCTTAGTCAACACCTT
TTCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAT
GGTGGTTCCGAATTAAGCCAAAA
   
Sequence Name 78-K116810-SALK_129208C-R210
GenBank Accession LN486196 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K116810-SALK_129208C-R210
TGTTGACTAGAAAGAAGAGCTTACCTCTAGCAAGAAGAGAGATGATTTGTATGACACGGA
TAGTAGTAGTATTCGAGCAGATTCAAGGTTAATCCCATTTACTCCGATATAGATGAAAAT
ATTCATATACTTAATGTGTTCTCACAAAACGTGTTGTTCACGTTTGATCATTGAAGGCTA
ATGTTTGATTGGTGTCTAATGGTTGAATGTAGATCAACGTCTTATGTTTCATCCGGACAT
GAACTACTTTCGGGAAGATCATTGCAACACCGTGGAAATTGGCACGCCGACTTGCTGGAT
TGTTGTTCAGAACCTTGTCTTTGTAAGCCCTCTCATTCTGTTT

Last Updated on 06.12.2018 16:11