Genotyping and primer details


Genotyping and primer details for SALK_129744c - At5g10060
Line ID SALK_129744c
Locus At5g10060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3146949 of line SALK_129744c Chr5:3146949
   
Insertion allele amplicon LBb3 - 65vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 65vi: 5'-GGATAGACCAGAAGTCTTTGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3146330
   
wt amplicon (over InsPos) 72vw - 65vi
wt amplicon start Chr5:3146330
wt amplicon end Chr5:3147481
wt amplicon size 1152 bp
Locus specific primer 65vi: 5'-GGATAGACCAGAAGTCTTTGTTGC-3'
2nd locus specific primer 72vw: 5'-TAAGGTTCTTGAGATGTGTGATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11