Genotyping and primer details


Genotyping and primer details for SALK_129775c - At5g55160
Line ID SALK_129775c
Locus At5g55160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22383814 of line SALK_129775c Chr5:22383814
   
Insertion allele amplicon LBb3 - 26hg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26hg: 5'-ATGAAAGCTTTTCGTTGCTATGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22384247
   
wt amplicon (over InsPos) 08ix - 26hg
wt amplicon start Chr5:22383328
wt amplicon end Chr5:22384247
wt amplicon size 920 bp
Locus specific primer 26hg: 5'-ATGAAAGCTTTTCGTTGCTATGAT-3'
2nd locus specific primer 08ix: 5'-TTGTGGAATACAGTGTTGAGAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11