Sequences from confirmation amplicon


Confirmation sequences for line SALK_129775c and hit At5g55160 are shown.
Line ID SALK_129775c
Locus At5g55160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22383814
Primers used for PCR 26hg - LBb3, show primer details
   
Sequence Name 84-K112669-SALK_129775C-26hg
GenBank Accession LN486272 [GenBank]
Sequencing primer gene specific primer, 26hg: 5'-ATGAAAGCTTTTCGTTGCTATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K112669-SALK_129775C-26hg
GCATTTTCGTTGTTGGTACTTGGTTGGTTATGATACAGTTTTTGGTATTGAGAAAATCGT
ATATAATACTCTTCTTGAAGTAACAATCATTGGTAATTTTTCTTGTGTAGGATGGTAATG
AAGTCTTCTTTAGGATCAAGAGAAGCACTCAGCTCAAAAAACTGATGAATGCTTACTGTG
ACCGTCAGTCTGTGGATTTCAACTCAATTGCTTTCTTGTTTGATGGTCGTCGTCTTCGTG
CCGAGCAGACTCCAGATGAGGTAACATCACTCTCTGTACCCCATTCTCTTTTACTGTTTT
CCGTTTTTCAAAGTTGTATGATTTCCTTAACCGAGAATCTTCAATTTTACAGCTTGAAAT
GGAAGATGGAGATGACACAAATTGTGGACAACTTAATGGCCTGGATATATTGTGGTGTAA
ACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCAAATCGGGCAAA
   
Sequence Name 74-K112668-SALK_129775C-R210
GenBank Accession LN486104 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K112668-SALK_129775C-R210
TTTGTTACCCACAATATATCCAGGCCATTAAGTTGTCCACAATTTGTGTCATCTCCATCT
TCCATTTCAAGCTGTAAAATTGAAGATTCTCGGTTAAGGAAATCATACAACTTTGAAAAA
CGGAAAACAGTAAAAGAGAATGGGGTACAGAGAGTGATGTTACCTCATCTGGAGTCTGCT
CGGCACGAAGACGACGACCATCAAACAAGAAAGCAATTGAGTTGAAATCCACAGACTGAC
GGTCACAGTAAGCATTCATCAGTTTTTTGAGCTGAGTGCTTCTCTTGATCCTAAAGAAGA
CTTCATTACCATCCTACACAAGAAAAATTACCAATGATTGTTACTTCAAGAAGAGTATTA
TATACGATTTTCTCAATACCAAAAACTGTATCTATAACCAACCAAGTACCAACAACGAAA
AATGCCTACCAATGGGTACAGAAACATAAGCAAAACAAATCATAGCAACGAAAAGCTTTC
A

Last Updated on 06.12.2018 16:11