Genotyping and primer details


Genotyping and primer details for SALK_129978c - At2g40820
Line ID SALK_129978c
Locus At2g40820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17038321 of line SALK_129978c Chr2:17038321
   
Insertion allele amplicon LBb3 - 27md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27md: 5'-AACAATGTCTGCTTGTATCACTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17037654
   
wt amplicon (over InsPos) 07nl - 27md
wt amplicon start Chr2:17037654
wt amplicon end Chr2:17038508
wt amplicon size 855 bp
Locus specific primer 27md: 5'-AACAATGTCTGCTTGTATCACTCG-3'
2nd locus specific primer 07nl: 5'-CTAGAGATCAGGGACCGGTATGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11