Genotyping and primer details


Genotyping and primer details for SALK_130157c - At5g58600
Line ID SALK_130157c
Locus At5g58600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23683907 of line SALK_130157c Chr5:23683907
   
Insertion allele amplicon LBb3 - 28rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28rb: 5'-CTTATTACCAAGACATGGACCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23684508
   
wt amplicon (over InsPos) 32rz - 28rb
wt amplicon start Chr5:23683560
wt amplicon end Chr5:23684508
wt amplicon size 949 bp
Locus specific primer 28rb: 5'-CTTATTACCAAGACATGGACCGTT-3'
2nd locus specific primer 32rz: 5'-CCAAACCCTATGAAAACTACAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11