Sequences from confirmation amplicon


Confirmation sequences for line SALK_130157c and hit At5g58600 are shown.
Line ID SALK_130157c
Locus At5g58600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23683907
Primers used for PCR 28rb - LBb3, show primer details
   
Sequence Name 84-K116055-SALK_130157C-28rb
GenBank Accession LN486275 [GenBank]
Sequencing primer gene specific primer, 28rb: 5'-CTTATTACCAAGACATGGACCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K116055-SALK_130157C-28rb
GCGTATTGGGTCGAACTCACGTTGATAGATCCCGAACACAAGTCTTGTTCCTCTCCATTT
CTCCACACACGACAAGTAACTCAGCCTCTCTTGTTATATTGGATCATATTTTCTGATGTT
TTTGAAATGAATGTCCTGACACCATTTGTATACATTTTGGGATTGATCAGCCCGAGTGAC
TGGGCGGCATCATCGTCTTCAGGATCCAAGAACTGCTACGGAGAAACAGAACCGATCACA
GGAACAGCTTATCCAGTGAGCTCCTACACAGATCAGCTAAGATCAGTGATTGTTGAAGTG
CTTCACGGGATGCACAATCCGGCGTTTCTTCTCGACATAACACTCCTCTCTTCCCTAAGA
AAAGACGGTCATCCGTCAGTATACAGCGGCCTCATTAGCGGTTCACAAAGGTCTAGACCA
GACCAGTCTGCAGATTGTAGCCATTGGTGTTTGCCTGGTTTACCTGATACATGGAACCAG
TTGTTGTATACGCTTCTCATCTATTAGCTTCTTTTTAAATCTCTTTCCATCAAATTATTC
GGTTAGTAATATTTTAACACATTCGAAGAGAGAATCTTAAGATTAATCACGGAATCAGTC
AATTGTATAAACTTAATAACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 57-K116054-SALK_130157C-R210
GenBank Accession LN485670 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K116054-SALK_130157C-R210
AACCTAATAATTTGATGGAAAGAGATTTAAAAATACGCTAATAGATGATAAGCGTATACA
GCGACTGGTTCCTTGTATCAAGTAAACCGGGCAAACACCGATGGCTACAATCTGCATACA
GGTCTGGTCTAGACCTTTGAAGAACCGCTGATGAGGCCGCTGTATACTGACGGATGACCG
TCTTTTCTTAGGGAAGAGAGGAGTGTTATGTCGAGAAAAAACGCCGGATTGTGCATCCCG
TGAAGCACTTCAACAATCACTGATCTTAGCTGATCTGTGTAGGAGCTCACTGGATAAGCT
GTTCCTGTGATCGGTTCTGTTTCTCCGTAGCAGTTCTTGGATCCTGAAGACGATGATGCC
GCCCAGTCACTCGGGCTGATCAATCCCAAAATGTATACAAATGGCGTCAGGACATTCATT
TCACAAACATCAGAAAATATGATCCAATATAACAAGAGAGGCTGACTTACTTGTCGTGTG
TTGGAGAAATGGAGAGGAACAATACTTGTGTTCGGGATCTATCAACGTGAGTTTCGACCC
AATACGCCCAAGTGCGAAGTGCTTTCTCCATTGCCACAAAACGGTCCATGTCTTGGTAAT
AAGAGGGGTTTTTCTTTT

Last Updated on 06.12.2018 16:11