Genotyping and primer details


Genotyping and primer details for SALK_130637c - At5g44700
Line ID SALK_130637c
Locus At5g44700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18033882 of line SALK_130637c Chr5:18033882
   
Insertion allele amplicon LBb3 - 41fb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 41fb: 5'-GAGAGATTCCTGTTGAGATTGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18034622
   
wt amplicon (over InsPos) 13go - 41fb
wt amplicon start Chr5:18033624
wt amplicon end Chr5:18034622
wt amplicon size 999 bp
Locus specific primer 41fb: 5'-GAGAGATTCCTGTTGAGATTGGAC-3'
2nd locus specific primer 13go: 5'-TGTTGGAATCAAGAAGCACATTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11