Sequences from confirmation amplicon


Confirmation sequences for line SALK_130637c and hit At5g44700 are shown.
Line ID SALK_130637c
Locus At5g44700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18033882
Primers used for PCR 41fb - LBb3, show primer details
   
Sequence Name 86-K111509-SALK_130637C-41fb
GenBank Accession LN486296 [GenBank]
Sequencing primer gene specific primer, 41fb: 5'-GAGAGATTCCTGTTGAGATTGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K111509-SALK_130637C-41fb
ACACAACTTTACCGGACGTATACCGTCTACGATTTCGACGTTACCTAAGCTTGAATCTCT
TGATCTGTCTCACAATCAGCTTGTAGGAGAAGTTCCTGGCCAAATCGGTGACATGAAAAG
CTTGGGATATCTTAACCTCTCTTACAACAACCTTGAGGGAAAGTTAAAGAAACAGTTCTC
TAGATGGCAAGCTGATGCGTTTGTAGGCAATGCAGGTCTTTGTGGAAGCCCTCTTAGTCA
TTGCAACAGAGCTGGATCAAAGAATCAACGAAGTCTTAGTCCGAAAACAGTGGTTATAAT
CTCTGCGATTTCATCATTAGCAGCGATTGCTTTGATGGTACTTGTTATCATCCTCTTCTT
CAAGCAAAACCATGATCTTTTCAAGAAAGTGCGAGGCGGAAACAGCGCATTTTCATCGAA
CTCTTCTTCTTCACAAGCTCCTCTATTTAGTAATGGAGGTGCAAAGTCAGATATAAAGTG
GGATGACATAATGGAAGCTACACATTACCTTAACGAAGAGTTCATGATTGGATCAGGAGG
GTCAGGAAAAGTTTACAAAGCGGAGTTAAAGAACGGCGAGACGATNGCTGTGAAGAAGAT
TCTTTGGAAAGATGATTTGATGTCAAACAAGAGCTTTAACAGAGAAGTTAAGACACTTGG
AACAATCAGACATAGACNTTAAATTGCACTTAATAACAAATTGACGCTNAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGG
   
Sequence Name 72-K111508-SALK_130637C-R210
GenBank Accession LN486055 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K111508-SALK_130637C-R210
CATTTGTATAAGTGCAATTAATGTCTATGTCTGATTGTTCCAGTGTCTTAACTTCTCTGT
TAAAGCTCTTGTTTGACATCAAATCATCTTTCCAAAGAATCTTCTTCACAGCTATCGTCT
CGCCGTTCTTTAACTCCGCTTTGTAAACTTTTCCTGACCCTCCTGATCCAATCATGAACT
CTTCGTTAAGGTAATGTGTAGCTTCCATTATGTCATCCCACTTTATATCTGACTTTGCAC
CTCCATTACTAAATAGAGGAGCTTGTGAAGAAGAAGAGTTCGATGAAAATGCGCTGTTTC
CGCCTCGCACTTTCTTGAAAAGATCATGGTTTTGCTTGAAGAAGAGGATGATAACAAGTA
CCATCAAAGCAATCGCTGCTAATGATGAAATCGCAGAGATTATAACCACTGTTTTCGGAC
TAAGACTTCGTTGATTCTTTGATCCAGCTCTGTTGCAATGACTAAGAGGGCTTCCACAAA
GACCTGCATTGCCTACAAACGCATCAGCTTGCCATCTAGAGAACTGTTTCTTTAACTTTC
CCTCAAGGTTGTTGTAAGAGAGGTTAAGATATCCCAAGCTTTTCATGTCACCGATTTGGC
CAGGAACTTCTCCTACAAGCTGATTGTGAGACAGATCAAGAGATTCAAGCTTAGGTAACG
TCGAAATCGTAGACGGTATACGTCCGGTAAAGTTGTTGTAGCTGAGATCTAAAGCACTTT
GAAGATCTTGCAGCTGTCCAATCTCAACAGNAATCTCT

Last Updated on 06.12.2018 16:11