Genotyping and primer details


Genotyping and primer details for SALK_130727 - At1g64000
Line ID SALK_130727
Locus At1g64000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23747686 of line SALK_130727 Chr1:23747686
   
Insertion allele amplicon LBb3 - 67us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 67us: 5'-AGGAAAGACTTAGGCAGTTGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23747206
   
wt amplicon (over InsPos) 37vw - 67us
wt amplicon start Chr1:23747206
wt amplicon end Chr1:23748239
wt amplicon size 1034 bp
Locus specific primer 67us: 5'-AGGAAAGACTTAGGCAGTTGAACA-3'
2nd locus specific primer 37vw: 5'-AAATAAAAGGCCCAAATTGTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11