Sequences from confirmation amplicon


Confirmation sequences for line SALK_130727 and hit At1g64000 are shown.
Line ID SALK_130727
Locus At1g64000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23747686
Primers used for PCR 67us - LBb3, show primer details
   
Sequence Name 35-K117668-SALK_130727C-R210
GenBank Accession LN485225 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117668-SALK_130727C-R210
CATTTGTTATATGATCACTATTATTGCTATTATTACTCACTAATCCTCCCATCTCTCCTC
CACCTCCTCGAAAGTTCTCCAGCTGAGCAGATAGATGATAACCTGCTGGAGTACAAGAAG
AAGAAGATGGACCTACATCGCCCGAAAAGATTAACGAAGGAGCCATCATCATTAATGTTT
TGTCATCTAAGGAAGAAAAAGGATTGTTGTTTTCGCCTTCTTCTAGGGTTAACATAGGAT
TTGTGTTGTCAACCCCTTCCATTGATTATGAAGCTTTCACTATCTTGCTAGATCTCTCTC
TCTCTCTCTTACTATATATATGTGTGTGTTTATGTAAATATATGTTTGCATAAGAGGAAG
AAGGAGAAGAGGAGAAGAAAGGTTATAAGGATATTTGTAGGAAAGAAAGAAGAAGAATGA
GTTAAAGGGTATAATATTGAAGCCAACTATATTCACCTAACTTTTCTGTTCAACTGCCTA
AGTCTTT
   
Sequence Name 72-K117669-SALK_130727C-67us
GenBank Accession LN486064 [GenBank]
Sequencing primer gene specific primer, 67us: 5'-AGGAAAGACTTAGGCAGTTGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K117669-SALK_130727C-67us
CCCTTTACTCATTCTTCTTCTTTCTTTCCTACAAATATCCTTATAACCTTTCTTCTCCTC
TTCTCCTTCTTCCTCTTATGCAAACATATATTTACATAAACACACACATATATATAGTAA
GAGAGAGAGAGAGAGATCTAGCAAGATAGTGAAAGCTTCATAATCAATGGAAGGGGTTGA
CAACACAAATCCTATGTTAACCCTAGAAGAAGGCGAAAACAACAATCCTTTTTCTTCCTT
AGATGACAAAACATTAATGATGATGGCTCCTTCGTTAATCTTTTCGGGCGATGTAGGTCC
ATCTTCTTCTTCTTGTACTCCAGCAGGTTATCATCTATCTGCTCAGCTGGAGAACTTTCG
AGGAGGTGGAGGAGAGATGGGAGGATTAGTGAGTAATAATAGCAATAATAGTGATCATAA
TAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCG

Last Updated on 06.12.2018 16:11