Genotyping and primer details


Genotyping and primer details for SALK_131997 - At1g32583
Line ID SALK_131997
Locus At1g32583 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11787784 of line SALK_131997 Chr1:11787784
   
Insertion allele amplicon LBb3 - 50wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50wu: 5'-TTTGAGATTGAAAAAGGGTATTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11787169
   
wt amplicon (over InsPos) 50xt - 50wu
wt amplicon start Chr1:11787169
wt amplicon end Chr1:11788364
wt amplicon size 1196 bp
Locus specific primer 50wu: 5'-TTTGAGATTGAAAAAGGGTATTGG-3'
2nd locus specific primer 50xt: 5'-AGTGGATAATTTGGCACGTTCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11