Sequences from confirmation amplicon


Confirmation sequences for line SALK_131997 and hit At1g32583 are shown.
Line ID SALK_131997
Locus At1g32583 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11787784
Primers used for PCR 50wu - LBb3, show primer details
   
Sequence Name 90-K119308-SALK_131997C-50wu
GenBank Accession LN486337 [GenBank]
Sequencing primer gene specific primer, 50wu: 5'-TTTGAGATTGAAAAAGGGTATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K119308-SALK_131997C-50wu
ATCCAAACTTCCATTTCTTAAGTTTCTTCTCATCTCTCCACCATGCATGTGTATTATACC
TCTATAAAAAACTCTATATCTACTAGAACAGAGAAGAAGCCTCCACATCTTTTGGCCTGT
GCATGAGAATGGAACATATCTACAAATTCCAACATTGGCTCTTCTTCATAGGTCTTGGAG
TGTTGCTGTCTCTTTCTCTGTGTAAGTTCTTGTCTAGAATTTCCCTCTTTTTCTCTCTCT
TTTTCTTCTTCTTATACTATACACCAATTCCATATTTGAATATTGTCAATGTTGCAGCTG
TGAAGGCCAATGACTTCGAAGGTTCCAATGATAGAAGAAGCATTGCTCTCAAGGCTAGAA
GCTTCGTTTCCATAAACGAAAACCGCACCGCTCTTTCCAGAAAGCTACTACTCTCTCCCG
GTAATTCATAGACACTGTTAGTGTTTGGCTTCACATGTTCTATTAACACTCTTTTATGTT
TTAGGATCAATCTCTTTGTTTTTTTATGTATTAAGAACCTTAAAGACAAAATTTCAGACA
TTGGCGATGGGACCAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAA
TGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACC
GCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAA
TCCTGTTTGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 42-K119307-SALK_131997C-R210
GenBank Accession LN485344 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K119307-SALK_131997C-R210
CATTTGGTCCATCGCCAATGTCTGAAATTTTGTCTTTAAGGTTCTTAATACATAAAAAAA
CAAAGAGATTGATCCTAAAACATAAAAGAGTGTTAATAGAACATGTGAAGCCAAACACTA
ACAGTGTCTATGAATTACCGGGAGAGAGTAGTAGCTTTCTGGAAAGAGCGGTGCGGTTTT
CGTTTATGGAAACGAAGCTTCTAGCCTTGAGAGCAATGCTTCTTCTATCATTGGAACCTT
CGAAGTCATTGGCCTTCACAGCTGCAACATTGACAATATTCAAATATGGAATTGGTGTAT
AGTATAAGAAGAAGAAAAAGAGAGAGAAAAAGAGGGAAATTCTAGACAAGAACTTACACA
GAGAAAGAGACAGCAACACTCCAAGACCTATGAAGAAGAGCCAATGTTGGAATTTGTAGA
TATGTTCCATTCTCATGCACAGGCCAAAAGATGTGGAGGCTTCTTCTCTGTTCTAGTAGA
TATAGAGTTTTTTATAGAGGTATAATACACATGCATGGTGGAGAGATGAGAAGAAACTTA
AGAAATGGAAGTTTGTGATTGCATGCATGTCTGCTGTTTCCATAAGGGTATGGACCAATA
CCCTTTTTCAATCTCAA

Last Updated on 06.12.2018 16:11