Genotyping and primer details


Genotyping and primer details for SALK_132560c - At5g56030
Line ID SALK_132560c
Locus At5g56030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22688710 of line SALK_132560c Chr5:22688710
   
Insertion allele amplicon LBb3 - 32hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32hd: 5'-GAAACAATAACCTTCTCGACCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22689012
   
wt amplicon (over InsPos) 20ir - 32hd
wt amplicon start Chr5:22688175
wt amplicon end Chr5:22689012
wt amplicon size 838 bp
Locus specific primer 32hd: 5'-GAAACAATAACCTTCTCGACCTTG-3'
2nd locus specific primer 20ir: 5'-CTGCTTTCTACAAGAGTCTCAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11