Genotyping and primer details


Genotyping and primer details for SALK_132819c - At1g49720
Line ID SALK_132819c
Locus At1g49720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18401673 of line SALK_132819c Chr1:18401673
   
Insertion allele amplicon LBb3 - CG81
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG81: 5'-ACTGAAGAATTTGGAAGGAAGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18400777
   
wt amplicon (over InsPos) 01lt - CG81
wt amplicon start Chr1:18400777
wt amplicon end Chr1:18401968
wt amplicon size 1192 bp
Locus specific primer CG81: 5'-ACTGAAGAATTTGGAAGGAAGTGA-3'
2nd locus specific primer 01lt: 5'-CTCAGCTTCCAGTTCCAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11