Sequences from confirmation amplicon


Confirmation sequences for line SALK_132819c and hit At1g49720 are shown.
Line ID SALK_132819c
Locus At1g49720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18401673
Primers used for PCR CG81 - LBb3, show primer details
   
Sequence Name 63-K107792-SALK_132819C-CG81
GenBank Accession LN485814 [GenBank]
Sequencing primer gene specific primer, CG81: 5'-ACTGAAGAATTTGGAAGGAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107792-SALK_132819C-CG81
GGGTCTCACATTGATATCAACAACTTAGGCGGCGATACTTCTAGAGGGAATGAGTCAAAG
CCATTGGCGAGGCAGTCTTCGTTATATTCCTTAACGTTTGATGAGCTTCAGAGCACATTA
GGTGAGCCGGGGAAAGATTTTGGGTCTATGAATATGGATGAGTTACTCAAGAACATATGG
ACTGCTGAGGATACTCAAGCCTTTATGACTACTACATCTTCGGTTGCAGCCCCGGGACCT
AGTGGTTTTGTTCCGGGAGGAAATGGTTTACAGAGGCAAGGCTCCTTGACCTTGCCTAGA
ACGCTTAGTCAGAAGACTGTCGATGAAGTCTGGAAATACCTGAATTCGAAAGAAGGTAGT
AATGGGAATACTGGAACGGATGCGCTTGAGAGGCAACAGACTTTAGGGGAAATGACTCTG
GAAGATTTCTTACTCCGTGCTGGCGTTGTTAAAGAAGATAATACTCAGCAGAACGAAAAC
AGTAGTAGCGGGTTTTATGCTAACAACGGTGCTGCTGGTTTGGAGTTTGGATTTGGTCAG
CCGAATCAAAACAGCATATCGTTCAACGGGAACAATAGTTCTATGATCATGAATCAAGCA
CCTGGTTTAGGCCTCAAAGTTGGTGGAACCATGCAGCAGCAGCAGCAGCCACATCAGCAG
CAGTTGCAGCAGCCACATCAGAGACTGCCTCCAACTATCTTTCCAAAACAAGCGAATGTA
ACATTTGCGGCGCCTGTAAATATGGTCAACAGGGGTTTATTTGAGACTAGCGCAGATGGT
CCAGCCAACAGTAATATGGGAGGAGCAGGGGGTACTGTTACAGCTACTTCTCCTGGGACG
AGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAAAACACATTGCGGA
CGTTTTTATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACC
   
Sequence Name 14-K107793-SALK_132819C-R210
GenBank Accession LN484658 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K107793-SALK_132819C-R210
GTCATTTGTTTACCCACAATATATCCTGCTCGTCCCAGGAGAAGTAGCTGTAACAGTACC
CCCTGCTCCTCCCATATTACTGTTGGCTGGACCATCTGCGCTAGTCTCAAATAAACCCCT
GTTGACCATATTTACAGGCGCCGCAAATGTTACATTCGCTTGTTTTGGAAAGATAGTTGG
AGGCAGTCTCTGATGTGGCTGCTGCAACTGCTGCTGATGTGGCTGCTGCTGCTGCTGCAT
GGTTCCACCAACTTTGAGGCCTAAACCAGGTGCTTGATTCATGATCATAGAACTATTGTT
CCCGTTGAACGATATGCTGTTTTGATTCGGCTGACCAAATCCAAACTCCAAACCAGCAGC
ACCGTTGTTAGCATAAAACCCGCTACTACTGTTTTCGTTCTGCTGAGTATTATCTTCTTT
AACAACGCCAGCACGGAGTAAGAAATCTTCCAGAGTCATTTCCCCTAAAGTCTGTTGCCT
CTCAAGCGCATCCGTTCCAGTATTCCCATTACTACCTTCTTTCGAATTCAGGTATTTCCA
GACTTCATCGACAGTCTTCTGACTAAGCGTTCTAGGCAAGGTCAAGGAGCCTTGCCTCTG
TAAACCATTTCCTCCCGGAACAAAACCACTAGGTCCCGGGGCTGCAACCGAAGATGTAGT
AGTCATAAAGGCTTGAGTATCCTCAGCAGTCCATATGTTCTTGAGTAACTCATCCATATT
CATAGACCCAAAATCTTTCCCCGGCTCACCTAATGTGCTCTGAAGCTCATCAAACGTTAA
GGAATATAACGAAGACTGCCTCGCCAATGGCTTTGACTCATTCCCTCTAGAAGTATCGCC
GCCTAAGTTGTTGATATCAATGTGAGTACCCATACTTCTTTTCTGTTTCACAACGGAATC
ACTTCCT

Last Updated on 06.12.2018 16:11