Genotyping and primer details


Genotyping and primer details for SALK_133220c - At1g49630
Line ID SALK_133220c
Locus At1g49630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18373197 of line SALK_133220c Chr1:18373197
   
Insertion allele amplicon LBb3 - 39ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39ea: 5'-CTCAGCTATTCTAGCTTTCAGGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18372235
   
wt amplicon (over InsPos) 23lt - 39ea
wt amplicon start Chr1:18372235
wt amplicon end Chr1:18373406
wt amplicon size 1172 bp
Locus specific primer 39ea: 5'-CTCAGCTATTCTAGCTTTCAGGGA-3'
2nd locus specific primer 23lt: 5'-TATTCTTTGGTACAGTGTGGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11