Genotyping and primer details


Genotyping and primer details for SALK_133523c - At5g40650
Line ID SALK_133523c
Locus At5g40650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16283256 of line SALK_133523c Chr5:16283256
   
Insertion allele amplicon LBb3 - 99by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 99by: 5'-CTCATCAGCTACAGTGTTTCTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16283692
   
wt amplicon (over InsPos) 30dz - 99by
wt amplicon start Chr5:16282656
wt amplicon end Chr5:16283692
wt amplicon size 1037 bp
Locus specific primer 99by: 5'-CTCATCAGCTACAGTGTTTCTTGC-3'
2nd locus specific primer 30dz: 5'-TTGTGCTTCTTCATCTGTCTCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11