Genotyping and primer details


Genotyping and primer details for SALK_133989 - At2g17240
Line ID SALK_133989
Locus At2g17240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7498546 of line SALK_133989 Chr2:7498546
   
Insertion allele amplicon R204 - BI62
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI62: 5'-TAGTTAATCAGAAATTGGCACACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7497580
   
wt amplicon (over InsPos) BI63 - BI62
wt amplicon start Chr2:7497580
wt amplicon end Chr2:7498690
wt amplicon size 1111 bp
Locus specific primer BI62: 5'-TAGTTAATCAGAAATTGGCACACG-3'
2nd locus specific primer BI63: 5'-TCACTAATCCCACCTAATCCAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11