Genotyping and primer details


Genotyping and primer details for SALK_134251c - At1g02660
Line ID SALK_134251c
Locus At1g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:572461 of line SALK_134251c Chr1:572461
   
Insertion allele amplicon LBb3 - 28md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 28md: 5'-CTGCAGAATTCTTGTGGTTAATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:572797
   
wt amplicon (over InsPos) 05nl - 28md
wt amplicon start Chr1:571844
wt amplicon end Chr1:572797
wt amplicon size 954 bp
Locus specific primer 28md: 5'-CTGCAGAATTCTTGTGGTTAATGA-3'
2nd locus specific primer 05nl: 5'-CAAGCAATCAGTGGTTACAAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11