Genotyping and primer details


Genotyping and primer details for SALK_135222c - At3g12060
Line ID SALK_135222c
Locus At3g12060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3843586 of line SALK_135222c Chr3:3843586
   
Insertion allele amplicon LBb3 - 04jd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04jd: 5'-TCCAAATGTACCAACAAAACAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3842797
   
wt amplicon (over InsPos) 15km - 04jd
wt amplicon start Chr3:3842797
wt amplicon end Chr3:3844067
wt amplicon size 1271 bp
Locus specific primer 04jd: 5'-TCCAAATGTACCAACAAAACAAAA-3'
2nd locus specific primer 15km: 5'-GTTTCTATTCAGCGAATCTCCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11