Genotyping and primer details


Genotyping and primer details for SALK_135734c - At1g56010
Line ID SALK_135734c
Locus At1g56010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20947873 of line SALK_135734c Chr1:20947873
   
Insertion allele amplicon LBb3 - 43ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43ux: 5'-TGCTCATTGATGATGTAGTGATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20947159
   
wt amplicon (over InsPos) 69vl - 43ux
wt amplicon start Chr1:20947159
wt amplicon end Chr1:20948061
wt amplicon size 903 bp
Locus specific primer 43ux: 5'-TGCTCATTGATGATGTAGTGATGA-3'
2nd locus specific primer 69vl: 5'-TCCCTCACGGATGTATCTTTTATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11