Genotyping and primer details


Genotyping and primer details for SALK_136293 - At1g66340
Line ID SALK_136293
Locus At1g66340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24735128 of line SALK_136293 Chr1:24735128
   
Insertion allele amplicon LBb3 - 29md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29md: 5'-AGGTGTGTGTATGTGTGAGAGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24734651
   
wt amplicon (over InsPos) 71vw - 29md
wt amplicon start Chr1:24735913
wt amplicon end Chr1:?
wt amplicon size 1263 bp
Locus specific primer 29md: unknown
2nd locus specific primer 71vw: 5'-TCAGTCTTTGTTCAGGGGTTAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11