Sequences from confirmation amplicon


Confirmation sequences for line SALK_136293 and hit At1g66340 are shown.
Line ID SALK_136293
Locus At1g66340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24735128
Primers used for PCR 29md - LBb3, show primer details
   
Sequence Name 70-K117945-SALK_136293C-29md
GenBank Accession LN486015 [GenBank]
Sequencing primer gene specific primer, 29md: 5'-AGGTGTGTGTATGTGTGAGAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117945-SALK_136293C-29md
CTATGGAGTCTGCATTGTATGAACCGCAATGGCCAGCGGATGAATTGTTAATGAAATACC
AATACATCTCCGATTTCTTCATTGCGATTGCGTATTTTTCGATTCCTCTTGAGTTGATTT
ACTTTGTGAAGAAATCAGCCGTGTTTCCGTATAGATGGGTACTTGTTCAGTTTGGTGCTT
TTATCGTTCTTTGTGGAGCAACTCATCTTATTAACTTATGGACTTTCACTACGCATTCGA
GAACCGTGGCGCTTGTGATGACTACCGCGAAGGTGTTAACCGCTGTTGTCTCGTGTGCTA
CTGCGTTGATGCTTGTTCATATTATTCCTGATCTTTTGAGTGTTAAGACTCGGGAGCTTT
TCTTGAAAAATAAAGCTGCTGAGCTCGATAGAGAAATGGGATTGATTCGAACTCAGGAAG
AAACCGGAAGAATGTACGTCCATTGCGGCTATTAATAACACATTGACAACTTAATAACAC
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCGAATCGGGCAAAAT
   
Sequence Name 35-K117944-SALK_136293C-R210
GenBank Accession LN485226 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117944-SALK_136293C-R210
AGTTGTCAATGTGTTATTAATAGCCGCAATGGACGTACATTCTTCCGGTTTCTTCCTGAG
TTCGAATCAATCCCATTTCTCTATCGAGCTCAGCAGCTTTATTTTTCAAGAAAAGCTCCC
GAGTCTTAACACTCAAAAGATCAGGAATAATATGAACAAGCATCAACGCAGTAGCACACG
AGACAACAGCGGTTAACACCTTCGCGGTAGTCATCACAAGCGCCACGGTTCTCGAATGCG
TAGTGAAAGTCCATAAGTTAATAAGATGAGTTGCTCCACAAAGAACGATAAAAGCACCAA
ACTGAACAAGTACCCATCTATACGGAAACACGGCTGATTTCTTCACAAAGTAAATCAACT
CAAGAGGAATCGAAAAATACGCAATCGCAATGAAGAAATCGGAGATGTATTGGTATTTCA
TTAACAATTCATCCGCTGGCCATTGCGGTTCAATACAATTGCAGACTTCCATTATGAATT
TTTTACACTATAGTTCCTCTCTCACACATACACACACCT

Last Updated on 06.12.2018 16:11