Genotyping and primer details


Genotyping and primer details for SALK_136827c - At2g38660
Line ID SALK_136827c
Locus At2g38660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16167204 of line SALK_136827c Chr2:16167204
   
Insertion allele amplicon LBb3 - 69hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69hw: 5'-CAGAGACTGAACAGAAGACTGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16166658
   
wt amplicon (over InsPos) 14jm - 69hw
wt amplicon start Chr2:16166658
wt amplicon end Chr2:16167388
wt amplicon size 731 bp
Locus specific primer 69hw: 5'-CAGAGACTGAACAGAAGACTGTGG-3'
2nd locus specific primer 14jm: 5'-CATCAACATCTTTCTCCAATCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11