Genotyping and primer details


Genotyping and primer details for SALK_137192c - At4g26180
Line ID SALK_137192c
Locus At4g26180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13260590 of line SALK_137192c Chr4:13260590
   
Insertion allele amplicon LBb3 - 18mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18mh: 5'-TCCTGAGAATCAACAATCTGTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13260010
   
wt amplicon (over InsPos) 09nl - 18mh
wt amplicon start Chr4:13260010
wt amplicon end Chr4:13260902
wt amplicon size 893 bp
Locus specific primer 18mh: 5'-TCCTGAGAATCAACAATCTGTGTC-3'
2nd locus specific primer 09nl: 5'-CTTTCTTTGGTTGCTTGTGTATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11