Genotyping and primer details


Genotyping and primer details for SALK_137492c - At1g20200
Line ID SALK_137492c
Locus At1g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7004380 of line SALK_137492c Chr1:7004380
   
Insertion allele amplicon LBb3 - 40ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40ea: 5'-ACAACCTAAAGCCAGATGCTAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7003709
   
wt amplicon (over InsPos) 01fw - 40ea
wt amplicon start Chr1:7003709
wt amplicon end Chr1:7004636
wt amplicon size 928 bp
Locus specific primer 40ea: 5'-ACAACCTAAAGCCAGATGCTAAAA-3'
2nd locus specific primer 01fw: 5'-ACGAAAATAAGTGAGAGAGGTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11