Genotyping and primer details


Genotyping and primer details for SALK_138712c - At3g57920
Line ID SALK_138712c
Locus At3g57920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21445431 of line SALK_138712c Chr3:21445431
   
Insertion allele amplicon LBb3 - 14fy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14fy: 5'-GTATTTGTTGGTGTCTGAAGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21444787
   
wt amplicon (over InsPos) 01gi - 14fy
wt amplicon start Chr3:21444787
wt amplicon end Chr3:21445876
wt amplicon size 1090 bp
Locus specific primer 14fy: 5'-GTATTTGTTGGTGTCTGAAGTTGC-3'
2nd locus specific primer 01gi: 5'-GAGTAAGAGGAAGCCAAAACCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11