Sequences from confirmation amplicon


Confirmation sequences for line SALK_138712c and hit At3g57920 are shown.
Line ID SALK_138712c
Locus At3g57920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21445431
Primers used for PCR 14fy - LBb3, show primer details
   
Sequence Name 69-K111413-SALK_138712C-14fy
GenBank Accession LN485975 [GenBank]
Sequencing primer gene specific primer, 14fy: 5'-GTATTTGTTGGTGTCTGAAGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K111413-SALK_138712C-14fy
AGCTTGAATCTGTGCTATTGTTGTTATCATCTCTGGACATGTAGTAAAGCTTGAGCTTCC
ATGTGATAAAACATTCATGTGTGGATGGGTTTCCCTAACTGGATTAATCTGCCACGGTCC
AGGCCGCTGCATCACTGATCTTGCGGTTGACCACGCAGTAGGATCTCCCAAAACGCTTTT
AATCATTGCAGCATTGGGGTTTCCTGACACACAGGAAATTCAATAACTTGTTTTTGAGAC
TCATTGAGCTGAAAAGGGCTGTGTATATTGGGATCTAACCGTAAAGAGATGGAGCGATTC
GAGAGTAATGAGAAGTGAAAAGAGCCGTTGTGGGTTGTGGTTTTCTTCGTCGTTCGTTAT
GACAAGCGAGTCTTCTGCGACAACTTCTTTTCTCCAAGTCAAACTCAGAAAGCTGGTGAA
ACCTGATTTGATATAAAGAAAAGAAAGCTTCAAAGAAAAGCAACCTAAATAATGCATAAG
ACAAGAGACAAGACCACATAATAACTAGCTGTAAAGTTTAAGTTTTGATAAGACTCTTCA
ATGACGTTAAGAGCCAAAACTCAAAAAGCTCAAAAGGTACTGGGGTGGTTTTTCAGGATA
TATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAA
ATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 64-K111412-SALK_138712C-R210
GenBank Accession LN485845 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K111412-SALK_138712C-R210
CATTTGTTACACCACAATATATCCTGAAAACCACCCCAGTACCTTTTGAGCTTTTCGATT
TTTGGCTCTTAACGTCATTGAAGAGTCTTATCAAAACTTAAACTTTACAGCTAGTTATTA
TGTGGTCTTGTCTCTTGTCTTATGCATTATTTAGGTTGCTTTTCTTTGAAGCTTTCTTTT
CTTTATATCAAATCAGGTTTCACCAGCTTTCTGAGTTTGACTTGGAGAAAAGAAGTTGTC
GCAGAAGACTCGCTTGTCATAACGAACGACGAAGAAAACCACAACCCACAACGGCTCTTT
TCACTTCTCATTACTCTCGAATCGCTCCATCTCTTTACGGTTAGATCCCAATATACACAG
CCCTTTTCAGCTCAATGAGTCTCAAAAACAAGTTATTGAATTTCCTGTGTGTCAGGAAAC
CCCAATGCTGCAATGATTAAAAGCGTTTTGGGAGATCCTACTGCGTGGTCAACCGCAAGA
TCAGTGATGCAGCGGCCTGGACCGTGGCAGATTAATCCAGTTAGGGAAACCCATCCACAC
ATGAATGTTTTATCACATGGAAGCTCAAGCTTTACTACATGTCCAGAGATGATAAACAAC
AATAGCACAGATTCAAGCTGTGCTCTCTCTCTTCTGTCAAACTCATACCCAATTCATCAG
CAGCAACTTCAGACACCAACAAATA

Last Updated on 06.12.2018 16:11