Genotyping and primer details


Genotyping and primer details for SALK_138829 - At3g19300
Line ID SALK_138829
Locus At3g19300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6692302 of line SALK_138829 Chr3:6692302
   
Insertion allele amplicon LBb3 - 70hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 70hw: 5'-TTGCAAAATTGAAGCAAAGTTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6692875
   
wt amplicon (over InsPos) 13jm - 70hw
wt amplicon start Chr3:6691978
wt amplicon end Chr3:6692875
wt amplicon size 898 bp
Locus specific primer 70hw: 5'-TTGCAAAATTGAAGCAAAGTTATG-3'
2nd locus specific primer 13jm: 5'-ATTGTCAAGTGATATGGATGGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11