Genotyping and primer details


Genotyping and primer details for SALK_139541c - At3g46600
Line ID SALK_139541c
Locus At3g46600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17159454 of line SALK_139541c Chr3:17159454
   
Insertion allele amplicon R204 - AD09
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AD09: 5'-GAGCTTTTGAGAAACTGAAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17158727
   
wt amplicon (over InsPos) 02am - AD09
wt amplicon start Chr3:17158727
wt amplicon end Chr3:17159686
wt amplicon size 960 bp
Locus specific primer AD09: 5'-GAGCTTTTGAGAAACTGAAGGAGA-3'
2nd locus specific primer 02am: 5'-TCACTATCTCTTTCCCGTCCTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11