Genotyping and primer details


Genotyping and primer details for SALK_139663c - At4g14070
Line ID SALK_139663c
Locus At4g14070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8114639 of line SALK_139663c Chr4:8114639
   
Insertion allele amplicon LBb3 - 38gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38gz: 5'-TCAACATGAATGGGTAAACTACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8113820
   
wt amplicon (over InsPos) 18ir - 38gz
wt amplicon start Chr4:8113820
wt amplicon end Chr4:8114768
wt amplicon size 949 bp
Locus specific primer 38gz: 5'-TCAACATGAATGGGTAAACTACCA-3'
2nd locus specific primer 18ir: 5'-TCATATTCACTTGTGGAGTTGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11