Genotyping and primer details


Genotyping and primer details for SALK_139887c - At5g06340
Line ID SALK_139887c
Locus At5g06340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1937052 of line SALK_139887c Chr5:1937052
   
Insertion allele amplicon LBb3 - 14gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14gj: 5'-CAGTTTGGTGATACACAGAGCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1937380
   
wt amplicon (over InsPos) 02hv - 14gj
wt amplicon start Chr5:1936747
wt amplicon end Chr5:1937380
wt amplicon size 634 bp
Locus specific primer 14gj: 5'-CAGTTTGGTGATACACAGAGCAAT-3'
2nd locus specific primer 02hv: 5'-GAATTTGTCCACTTGATTTGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11