Genotyping and primer details


Genotyping and primer details for SALK_140244 - At4g15096
Line ID SALK_140244
Locus At4g15096 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8622048 of line SALK_140244 Chr4:8622048
   
Insertion allele amplicon LBb3 - 47wj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 47wj: 5'-CATCTCATCGACATCATTTGTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8623051
   
wt amplicon (over InsPos) 07wy - 47wj
wt amplicon start Chr4:8621490
wt amplicon end Chr4:8623051
wt amplicon size 1562 bp
Locus specific primer 47wj: 5'-CATCTCATCGACATCATTTGTTCT-3'
2nd locus specific primer 07wy: 5'-GACAATGATTCAATGTCCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11