Sequences from confirmation amplicon


Confirmation sequences for line SALK_140244 and hit At4g15096 are shown.
Line ID SALK_140244
Locus At4g15096 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8622048
Primers used for PCR 47wj - LBb3, show primer details
   
Sequence Name 21-K118627-SALK_140244C-47wj
GenBank Accession LN484898 [GenBank]
Sequencing primer gene specific primer, 47wj: 5'-CATCTCATCGACATCATTTGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118627-SALK_140244C-47wj
TACGAAATGAATGCTTCGATTATGAAAATGACAGATTGATACAACGTTCATGAAACGCGA
TATCAACTTCTCATCTACAAGAGTTCAACATAAAACTCCGGCGAACGATGAGGAACGATT
CTCAGAGAGAATCCAAAAGGAAACTATAAACAACAAATCCAAAATCTTCTTATCTTCTGC
AAATGATCTTCATTAGCTATATATCTCTGCATCTCGATTCTTCCTCTAACACTCCCATCG
ACCAATCTTCTCTTGCCACGTCTCACAGCTACTGTCTCCACTCATGTATTCCACGTATCT
AACCATATATCAATGCCTAGTTTCCTATATCAAAACTCCCCTGTTGGAAGAACCTTGACC
ACAAGGTTCAGTTCGAATAGGTATTCTCAAGTAGCTCTTGTGATTCAATTCCAATCCCAG
GGTCAAAGGTTTGGCTCTGTATGACTTCAAGCTTTCGTTCGTCTTTGGCAAATTTCAGTT
TAACAAGCTGATACACACGTTGAGACACCTCTACAACATTCTTGGCCTCACTTCCAAGCT
TGTAAACAACCAATCTCCTGCTGTAAAAGAGCTGCGTCCCTTTGTCACTCTGTGTCCAAA
TAGAATATTCATAATGCCCAACACCAATTGATGAAACGGAGTGTATGAACTTAGTTCTCC
ATGTATGCTTTATTTGGAACCTCTCATCACTAAGCAGAAACGGTTTTATTAGCAATGCAT
CATGTCGCAGCCATAAGAGAAGTTGTCTAGAAAATGACTGAAAGAGTCTAAATCTCCCCT
TTTTCTTCAAGTTAAACATCATGTCCCTATACCCAAGCAAAATATGAACAAGTTCTGCCT
CCTTGCCGATCAAGAAACAACATGGATACTCTGTAGAAACCTTTTGCTTCATTTTGACTT
CAAAAACATATTGTAAAGCTCTTGGTACAACTCTATGTTTGAACTTGATCTCCT
   
Sequence Name 10-K118626-SALK_140244C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118626-SALK_140244C-R210
ATTTCTCATCGGGAAATGCTGCCAAATCTT

Last Updated on 06.12.2018 16:11