Genotyping and primer details


Genotyping and primer details for SALK_140255c - At3g54800
Line ID SALK_140255c
Locus At3g54800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20288039 of line SALK_140255c Chr3:20288039
   
Insertion allele amplicon LBb3 - 05et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05et: 5'-GAGCTTTTGAATTTCAGGTTTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20287407
   
wt amplicon (over InsPos) 09gc - 05et
wt amplicon start Chr3:20287407
wt amplicon end Chr3:20288277
wt amplicon size 871 bp
Locus specific primer 05et: 5'-GAGCTTTTGAATTTCAGGTTTACG-3'
2nd locus specific primer 09gc: 5'-ATAAGTTCCAGCTTCTCCAATCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11