Genotyping and primer details


Genotyping and primer details for SALK_140393c - At2g43010
Line ID SALK_140393c
Locus At2g43010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17888200 of line SALK_140393c Chr2:17888200
   
Insertion allele amplicon LBb3 - 21ay
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21ay: 5'-AAACATTTTACCAAAATCGTCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17888630
   
wt amplicon (over InsPos) 13cd - 21ay
wt amplicon start Chr2:17887633
wt amplicon end Chr2:17888630
wt amplicon size 998 bp
Locus specific primer 21ay: 5'-AAACATTTTACCAAAATCGTCGTT-3'
2nd locus specific primer 13cd: 5'-CATGATCGAAGCAAAAACATAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11