Genotyping and primer details

Genotyping and primer details for SALK_140623c - At2g24420
Line ID SALK_140623c
Locus At2g24420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10380065 of line SALK_140623c Chr2:10380065
Insertion allele amplicon LBb3 - 06et
Locus specific primer 06et: 5'-TCTGAATGTCAGATTGATGTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10379483
wt amplicon (over InsPos) 18gc - 06et
wt amplicon start Chr2:10379483
wt amplicon end Chr2:10380399
wt amplicon size 917 bp
Locus specific primer 06et: 5'-TCTGAATGTCAGATTGATGTCTCC-3'
2nd locus specific primer 18gc: 5'-TTGAGAAATTCACACAAGCCATAG-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11