Genotyping and primer details


Genotyping and primer details for SALK_141625c - At3g44110
Line ID SALK_141625c
Locus At3g44110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15869322 of line SALK_141625c Chr3:15869322
   
Insertion allele amplicon LBb3 - 30md
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30md: 5'-CAGAAGATCACATTTGAAGGACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15869869
   
wt amplicon (over InsPos) 03nl - 30md
wt amplicon start Chr3:15869002
wt amplicon end Chr3:15869869
wt amplicon size 868 bp
Locus specific primer 30md: 5'-CAGAAGATCACATTTGAAGGACAA-3'
2nd locus specific primer 03nl: 5'-CTTAAACCGTACCAAACTCCCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11